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These are the user uploaded subtitles that are being translated: 1 00:00:00,270 --> 00:00:03,020 (dramatic music) 2 00:00:04,910 --> 00:00:07,890 It's famous for its grizzly bears, 3 00:00:07,890 --> 00:00:12,890 its wolves, bison and its geothermal activity. 4 00:00:15,700 --> 00:00:17,200 All of greater Yellowstone 5 00:00:17,200 --> 00:00:20,943 is actually still an active volcano today. 6 00:00:23,040 --> 00:00:26,030 The geysers, hot springs and thermal pools 7 00:00:26,030 --> 00:00:30,151 signal the unrest that continues just below the surface. 8 00:00:30,151 --> 00:00:32,280 (dramatic music) 9 00:00:32,280 --> 00:00:36,710 There's a lot of volcanic activity underground 10 00:00:36,710 --> 00:00:41,710 beneath the GYE and so that expresses itself 11 00:00:41,760 --> 00:00:44,193 in many different ways at the surface. 12 00:00:45,240 --> 00:00:47,450 For many years scientists believed 13 00:00:47,450 --> 00:00:50,580 these pools were devoid of life, 14 00:00:50,580 --> 00:00:54,030 but a fantastic alien world exists 15 00:00:54,030 --> 00:00:56,343 in the waters of Yellowstone's hotspots. 16 00:00:57,540 --> 00:01:01,030 Yellowstone was the first place these were discovered. 17 00:01:01,030 --> 00:01:03,210 The thermal pools are filled with clues 18 00:01:03,210 --> 00:01:04,820 about the earliest life on earth 19 00:01:05,750 --> 00:01:08,103 and even life on distant planets. 20 00:01:09,320 --> 00:01:14,320 We study modern organisms that represent ancient life, 21 00:01:16,100 --> 00:01:19,330 or that give us an idea of where alien life 22 00:01:19,330 --> 00:01:22,639 {\an8}might be able to exist and how it could exist. 23 00:01:22,639 --> 00:01:25,389 {\an8}(dramatic music) 24 00:01:30,187 --> 00:01:33,354 (gentle bright music) 25 00:01:35,780 --> 00:01:39,240 In 1966, scientists discovered organisms 26 00:01:39,240 --> 00:01:41,730 in Yellowstone's boiling hot springs 27 00:01:41,730 --> 00:01:43,717 and called them thermophiles. 28 00:01:45,190 --> 00:01:48,680 A very famous microbiologist started sampling, 29 00:01:48,680 --> 00:01:52,240 {\an8}started doing standard microbiological tests 30 00:01:52,240 --> 00:01:55,430 {\an8}but at higher temperatures and really, really discovered 31 00:01:55,430 --> 00:01:57,983 that organisms were living in these hot springs. 32 00:02:00,870 --> 00:02:03,030 These thermophiles are thriving 33 00:02:03,030 --> 00:02:05,170 in an environment that would be fatal 34 00:02:05,170 --> 00:02:06,813 to most other forms of life. 35 00:02:07,990 --> 00:02:10,860 Thermal pools in and around Yellowstone 36 00:02:10,860 --> 00:02:14,270 are a living laboratory where scientists are even able 37 00:02:14,270 --> 00:02:18,260 to research potential life on distant planets. 38 00:02:18,260 --> 00:02:20,590 Understanding sort of the distribution 39 00:02:20,590 --> 00:02:24,440 of microbial life in extreme environments on our planet 40 00:02:24,440 --> 00:02:27,650 {\an8}gives us an idea of potentially where else 41 00:02:27,650 --> 00:02:30,533 {\an8}they could exist on other planetary bodies. 42 00:02:32,420 --> 00:02:34,110 Montana State University's 43 00:02:34,110 --> 00:02:38,910 Thermal Biology Institute studies bacteria, archaea, 44 00:02:38,910 --> 00:02:42,273 and viruses that hide in the springs and pools. 45 00:02:44,730 --> 00:02:46,720 One of the pools they're investigating 46 00:02:46,720 --> 00:02:48,413 is LaDuke Hot Springs. 47 00:02:50,890 --> 00:02:51,960 The field work here, 48 00:02:51,960 --> 00:02:55,650 there's a lot of unique safety issues. 49 00:02:55,650 --> 00:02:59,240 Stepping into a hot springs would be very dangerous, 50 00:02:59,240 --> 00:03:01,463 you can burn yourself very badly. 51 00:03:03,060 --> 00:03:05,740 It's so dangerous that we can't show 52 00:03:05,740 --> 00:03:07,893 how they actually collect their samples. 53 00:03:09,240 --> 00:03:12,870 It's definitely a case of don't try this at home. 54 00:03:12,870 --> 00:03:15,400 We are not going in to these hot springs 55 00:03:15,400 --> 00:03:20,400 with big shovels and digging up truck fulls of material. 56 00:03:20,730 --> 00:03:23,950 We're taking the smallest possible samples that we can 57 00:03:23,950 --> 00:03:26,163 to get the analysis that we wanna get. 58 00:03:27,400 --> 00:03:29,890 Brent is like a bio prospector 59 00:03:29,890 --> 00:03:32,513 as he investigates hot spring biology. 60 00:03:33,490 --> 00:03:36,830 In the pools there's a very large number 61 00:03:36,830 --> 00:03:39,900 of different organisms, so multiple species 62 00:03:39,900 --> 00:03:42,460 of bacteria and archaea. 63 00:03:42,460 --> 00:03:46,050 And then each of those has probably 64 00:03:46,050 --> 00:03:50,253 five or 10 viruses that infect those. 65 00:03:52,090 --> 00:03:53,900 They can provide clues as to 66 00:03:53,900 --> 00:03:57,250 how organisms lived long, long ago 67 00:03:57,250 --> 00:03:59,463 and what types of metabolisms they had. 68 00:04:00,620 --> 00:04:03,950 The rainbow-like colors seen in many areas 69 00:04:03,950 --> 00:04:06,013 come from heat-loving bacteria. 70 00:04:07,120 --> 00:04:09,100 So the photosynthetic organisms 71 00:04:09,100 --> 00:04:12,720 give you the greens and the bright oranges. 72 00:04:12,720 --> 00:04:15,470 Some yellow come from sulfur precipitation 73 00:04:15,470 --> 00:04:17,280 caused by the microbes. 74 00:04:17,280 --> 00:04:19,040 Some of the orange is minerals, 75 00:04:19,040 --> 00:04:21,030 you can get different colored minerals as well. 76 00:04:21,030 --> 00:04:23,400 So it's basically microbes and minerals 77 00:04:23,400 --> 00:04:26,200 interacting in the hot springs that give you the colors. 78 00:04:27,340 --> 00:04:29,470 Brilliant blue in the center 79 00:04:29,470 --> 00:04:32,950 comes from clear water reflecting back blue light, 80 00:04:32,950 --> 00:04:34,143 just like the sky. 81 00:04:35,100 --> 00:04:38,700 The wild, chromatic beauty attracts tourists 82 00:04:38,700 --> 00:04:40,680 but it also draws scientists 83 00:04:40,680 --> 00:04:43,570 that believe these extreme thermal conditions 84 00:04:43,570 --> 00:04:46,403 might replicate those found on other planets. 85 00:04:48,200 --> 00:04:51,860 Dr. Luke McKay is a microbial ecologist 86 00:04:51,860 --> 00:04:53,573 and an astrobiologist. 87 00:04:55,430 --> 00:04:57,710 I'm looking for organisms with 88 00:04:57,710 --> 00:05:00,820 what I call early evolved metabolisms, 89 00:05:00,820 --> 00:05:03,320 metabolisms that originated long, long ago 90 00:05:03,320 --> 00:05:05,433 shortly after the origin of life. 91 00:05:07,450 --> 00:05:08,980 Brent and Luke know 92 00:05:08,980 --> 00:05:11,380 that the invisible microbes that they hunt 93 00:05:11,380 --> 00:05:13,743 are elusive and diverse. 94 00:05:14,810 --> 00:05:17,930 When we show up at a thermal environment, 95 00:05:17,930 --> 00:05:19,620 typically the first thing we do 96 00:05:19,620 --> 00:05:24,138 is measure the temperature and the pH of the system. 97 00:05:24,138 --> 00:05:25,580 8.14. 98 00:05:25,580 --> 00:05:27,540 Depending on the environment and the type 99 00:05:27,540 --> 00:05:29,260 of thermal feature it is, 100 00:05:29,260 --> 00:05:32,410 we'll take different types of samples for chemical analysis 101 00:05:32,410 --> 00:05:35,430 to understand what types of chemical molecules, 102 00:05:35,430 --> 00:05:39,720 what types of chemistry is the hot spring composed of, 103 00:05:39,720 --> 00:05:41,280 because that will determine 104 00:05:41,280 --> 00:05:43,823 what types of microbes live in the hot spring. 105 00:05:45,500 --> 00:05:48,630 Both bacteria and archaea inhabit the pools 106 00:05:48,630 --> 00:05:52,683 in different proportions and both target viruses. 107 00:05:53,830 --> 00:05:55,830 And so, depending on the hot spring 108 00:05:55,830 --> 00:05:57,880 and the chemistry of the hot spring, 109 00:05:57,880 --> 00:05:59,500 the bacteria or archaea 110 00:05:59,500 --> 00:06:02,250 will use different chemistry, and so, 111 00:06:02,250 --> 00:06:04,250 if you have one hot spring that supports 112 00:06:04,250 --> 00:06:06,400 the metabolism of a lot of different archaea 113 00:06:06,400 --> 00:06:08,990 then you'll have a lot of archaea there. 114 00:06:08,990 --> 00:06:11,020 And pretty much anywhere where you're gonna have 115 00:06:11,020 --> 00:06:12,510 a lot of archaea and bacteria 116 00:06:12,510 --> 00:06:15,020 you're also gonna have a lot of viruses, 117 00:06:15,020 --> 00:06:17,483 and they're gonna be in any of the hot springs. 118 00:06:19,710 --> 00:06:21,160 These microbes are all part 119 00:06:21,160 --> 00:06:22,343 of the tree of life. 120 00:06:23,180 --> 00:06:25,480 All life on earth can be categorized 121 00:06:25,480 --> 00:06:28,120 {\an8}into one of the three domains of life. 122 00:06:28,120 --> 00:06:31,443 {\an8}Those are archaea, bacteria and eurkya. 123 00:06:32,420 --> 00:06:35,330 And bacteria, on the tree of life, 124 00:06:35,330 --> 00:06:39,890 is easily the most diverse of the three domains. 125 00:06:39,890 --> 00:06:43,750 Archaea is smaller and eurkya is even smaller than that 126 00:06:43,750 --> 00:06:47,290 in terms of, you know, if you can imagine an actual tree 127 00:06:47,290 --> 00:06:49,490 it would be like most of the branches 128 00:06:49,490 --> 00:06:51,730 are taken up by bacteria. 129 00:06:51,730 --> 00:06:53,450 It's crazy because when we hear 130 00:06:53,450 --> 00:06:55,450 what organisms are in the main carrier, 131 00:06:55,450 --> 00:06:57,490 we think it's insanely diverse, 132 00:06:57,490 --> 00:06:59,020 because it's us, it's humans, 133 00:06:59,020 --> 00:07:01,310 it's all the animals, insects, 134 00:07:01,310 --> 00:07:05,240 it's all the plants, all the fungi, protists, 135 00:07:05,240 --> 00:07:07,179 but in terms of their DNA, it's actually 136 00:07:07,179 --> 00:07:11,380 a very small fraction of the overall diversity of life. 137 00:07:11,380 --> 00:07:13,160 Most of that diversity is captured 138 00:07:13,160 --> 00:07:14,803 within the microbial world. 139 00:07:17,160 --> 00:07:18,920 Single celled archaea 140 00:07:18,920 --> 00:07:21,730 were originally classified with bacteria, 141 00:07:21,730 --> 00:07:23,633 but now have a branch of their own. 142 00:07:25,430 --> 00:07:27,660 Bacteria and archaea are different. 143 00:07:27,660 --> 00:07:29,750 They're branches of life 144 00:07:29,750 --> 00:07:34,633 that separated many, many billions of years ago. 145 00:07:35,850 --> 00:07:37,030 But then, when when we started 146 00:07:37,030 --> 00:07:39,370 to get molecular information, 147 00:07:39,370 --> 00:07:42,277 genomic or genetic information, in sequencing genes 148 00:07:42,277 --> 00:07:45,058 and comparing genes, we started to realize 149 00:07:45,058 --> 00:07:49,090 that bacteria and archaea are incredibly different 150 00:07:49,090 --> 00:07:50,150 from one another. 151 00:07:50,150 --> 00:07:52,973 Their genes are very dissimilar in many cases. 152 00:07:53,910 --> 00:07:56,350 This investigation requires extensive time 153 00:07:56,350 --> 00:07:59,060 in the field and in the lab. 154 00:07:59,060 --> 00:08:00,970 In TBI we're working on 155 00:08:00,970 --> 00:08:04,000 a couple of different aspects of thermophiles. 156 00:08:04,000 --> 00:08:05,760 {\an8}One is very fundamental. 157 00:08:05,760 --> 00:08:08,920 {\an8}So we're taking samples from the hot springs 158 00:08:08,920 --> 00:08:11,340 {\an8}and we've got groups of organisms 159 00:08:11,340 --> 00:08:13,500 {\an8}that no one's ever grown in a laboratory, 160 00:08:13,500 --> 00:08:15,690 {\an8}and nobody knows how to grow them. 161 00:08:15,690 --> 00:08:18,910 {\an8}We find them very common in the hot springs 162 00:08:18,910 --> 00:08:22,520 {\an8}and can't figure out how to reproduce that in the lab. 163 00:08:22,520 --> 00:08:26,100 {\an8}And so we're doing everything we can 164 00:08:26,100 --> 00:08:28,533 to learn about those micro organisms. 165 00:08:29,740 --> 00:08:33,530 If we can grow organisms in the lab we can understand 166 00:08:33,530 --> 00:08:35,640 so much about what they're doing 167 00:08:35,640 --> 00:08:37,620 and you can formulate hypothesis based 168 00:08:37,620 --> 00:08:40,300 on what you see in the hot spring and then test those 169 00:08:40,300 --> 00:08:42,070 in the tube in the lab. 170 00:08:42,070 --> 00:08:44,570 I take a sample from a hot spring 171 00:08:44,570 --> 00:08:48,920 and I extract all the DNA of all the genomes, 172 00:08:48,920 --> 00:08:52,130 of all the organisms, all the microorganisms 173 00:08:52,130 --> 00:08:54,580 in that hot spring sample. 174 00:08:54,580 --> 00:08:57,463 And then I sequence that DNA. 175 00:08:58,330 --> 00:09:01,590 And then the next step is the computer analysis 176 00:09:01,590 --> 00:09:04,850 of trying to understand how to 177 00:09:04,850 --> 00:09:06,990 take all of that genomic information 178 00:09:06,990 --> 00:09:10,863 and isolate different individual microbes. 179 00:09:12,420 --> 00:09:14,910 And then there are the strange viruses 180 00:09:14,910 --> 00:09:17,403 found in the streaming geysers and pools. 181 00:09:18,480 --> 00:09:21,080 You have thermophilic bacteria, 182 00:09:21,080 --> 00:09:23,440 you also have thermophilic archaea 183 00:09:23,440 --> 00:09:25,290 that both live in these hot springs, 184 00:09:25,290 --> 00:09:27,450 and then you have the viruses that infect 185 00:09:27,450 --> 00:09:29,530 both the archaea and the bacteria 186 00:09:29,530 --> 00:09:30,980 that live in the hot springs. 187 00:09:32,450 --> 00:09:35,350 Like bacteria, the word virus 188 00:09:35,350 --> 00:09:38,830 often conjures up images of sickness and death. 189 00:09:38,830 --> 00:09:41,700 But none of the heat loving viruses in Yellowstone 190 00:09:41,700 --> 00:09:45,283 affect our health because our bodies are too cold. 191 00:09:46,960 --> 00:09:50,820 So the viruses in the springs are dependent 192 00:09:50,820 --> 00:09:53,550 on those specific bacteria or archaea, 193 00:09:53,550 --> 00:09:56,923 so they don't infect humans. 194 00:09:58,120 --> 00:09:59,070 All have evolved 195 00:09:59,070 --> 00:10:02,790 from the earliest forms of life on embryonic earth. 196 00:10:02,790 --> 00:10:05,340 But together they suggest that life 197 00:10:05,340 --> 00:10:08,593 may have arisen in super hot environments. 198 00:10:09,900 --> 00:10:13,000 How did life originate here on earth? 199 00:10:13,000 --> 00:10:16,420 And what was the evolutionary history of life? 200 00:10:16,420 --> 00:10:19,070 How did life colonize certain environments 201 00:10:19,070 --> 00:10:23,300 that are very extreme or distinct from other environments? 202 00:10:23,300 --> 00:10:26,150 Some archaea, bacteria and viruses 203 00:10:26,150 --> 00:10:29,330 in extreme environments still live in the same way 204 00:10:29,330 --> 00:10:32,220 as our earliest microbial ancestors 205 00:10:32,220 --> 00:10:34,463 and use the same sources of energy. 206 00:10:35,740 --> 00:10:38,630 We oftentimes find organisms, microbes, 207 00:10:38,630 --> 00:10:42,950 with early evolved or deep evolutionary roots. 208 00:10:42,950 --> 00:10:44,810 In other words, they branch off 209 00:10:44,810 --> 00:10:48,590 at a very, very early point on the tree of life. 210 00:10:48,590 --> 00:10:51,250 And so these are the types of organisms 211 00:10:51,250 --> 00:10:54,450 that are likely, they're not the same 212 00:10:54,450 --> 00:10:57,810 as what was existing four billion years ago, 213 00:10:57,810 --> 00:11:01,390 but they may be more similar to that than anything else. 214 00:11:01,390 --> 00:11:05,960 And so if we understand their sort of modern day cousins 215 00:11:05,960 --> 00:11:07,840 of those ancient organisms, 216 00:11:07,840 --> 00:11:10,623 we can understand more about those ancient organisms. 217 00:11:11,480 --> 00:11:12,313 A new virus 218 00:11:12,313 --> 00:11:15,930 found in Yellowstone's thermal pools by Rebecca Hochstein 219 00:11:15,930 --> 00:11:19,140 and Martin Lawrence has a structure so ancient 220 00:11:19,140 --> 00:11:21,700 that scientists think it may be connected 221 00:11:21,700 --> 00:11:24,690 to the root of the universal tree of life 222 00:11:24,690 --> 00:11:26,270 according to a new study published 223 00:11:26,270 --> 00:11:28,163 by the National Academy of Sciences. 224 00:11:29,410 --> 00:11:32,830 It affects only archaea and it's spindle shaped. 225 00:11:32,830 --> 00:11:35,743 It seems to be part of a rare superfamily. 226 00:11:37,240 --> 00:11:40,350 Every time we go and sample, we find DNA 227 00:11:40,350 --> 00:11:43,230 from organisms that nobody's ever grown, 228 00:11:43,230 --> 00:11:44,920 nobody's ever named. 229 00:11:44,920 --> 00:11:48,720 And so just the diversity of unknown organisms out there 230 00:11:48,720 --> 00:11:51,080 is to me the most exciting. 231 00:11:51,080 --> 00:11:52,140 This is actually like 232 00:11:52,140 --> 00:11:54,850 a very active area of research. 233 00:11:54,850 --> 00:11:57,600 And as new genomic information comes in 234 00:11:57,600 --> 00:12:00,630 on different organisms, we are able to sequence new genomes 235 00:12:00,630 --> 00:12:03,370 and we're able to get new sequences to compare 236 00:12:03,370 --> 00:12:05,840 and to place on the tree of life. 237 00:12:05,840 --> 00:12:07,910 New branches start to arise. 238 00:12:07,910 --> 00:12:10,950 We start to actually discover new branches of life 239 00:12:10,950 --> 00:12:12,600 that we didn't know about before. 240 00:12:13,750 --> 00:12:15,890 The possibilities have experts 241 00:12:15,890 --> 00:12:17,493 in many fields excited. 242 00:12:19,530 --> 00:12:22,100 The main goal of my research 243 00:12:22,100 --> 00:12:26,290 is to understand what organisms are in these hot springs, 244 00:12:26,290 --> 00:12:29,930 but also as an engineer, what can we do with these, 245 00:12:29,930 --> 00:12:32,290 how can they benefit society? 246 00:12:32,290 --> 00:12:35,180 How can we use them to do 247 00:12:35,180 --> 00:12:40,180 any sort of biotechnological task that we set at hand? 248 00:12:41,150 --> 00:12:42,680 As they investigate the ways 249 00:12:42,680 --> 00:12:46,060 that life likely evolved they're inching closer 250 00:12:46,060 --> 00:12:48,723 to finding new places where life might be found. 251 00:12:50,070 --> 00:12:51,820 So as we start to gather data 252 00:12:51,820 --> 00:12:55,380 about the chemistry of Mars or the chemistry of Titan, 253 00:12:55,380 --> 00:12:58,570 or Enceladus or Europa, 254 00:12:58,570 --> 00:13:01,670 all of these exoplanets in our own solar system 255 00:13:01,670 --> 00:13:03,900 that are interesting places where life 256 00:13:03,900 --> 00:13:08,160 may have at least at one point colonized, 257 00:13:08,160 --> 00:13:12,090 then we can understand a lot about those environments 258 00:13:12,090 --> 00:13:13,690 by studying environments on Earth 259 00:13:13,690 --> 00:13:16,443 that are sort of exotic and interesting. 260 00:13:17,970 --> 00:13:20,470 Yellowstone's thermal pools may hold the keys 261 00:13:20,470 --> 00:13:23,823 to the origin of life on our planet and beyond. 262 00:13:25,400 --> 00:13:28,170 There is so much we don't know 263 00:13:28,170 --> 00:13:30,970 and that we're still learning constantly. 264 00:13:30,970 --> 00:13:33,000 Perhaps one day soon 265 00:13:33,000 --> 00:13:35,760 Yellowstone's thermophiles would reveal the answers 266 00:13:35,760 --> 00:13:38,193 to one of the most burning questions in science. 267 00:13:39,190 --> 00:13:42,130 Are we alone in the universe? 268 00:13:42,130 --> 00:13:47,130 I have no evidence to say that we are not alone. 269 00:13:48,530 --> 00:13:50,960 I have not discovered alien life, 270 00:13:50,960 --> 00:13:54,610 but I think it is incredibly probable 271 00:13:54,610 --> 00:13:55,873 that we are not alone. 272 00:13:58,001 --> 00:14:00,584 (bright music) 21232

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